Human cardiac troponin I (cTn-I) ELISA Kit | cTn-I elisa kit
Human cardiac troponin I (cTn-I) ELISA Kit
Reactivity
Human
Synonyms
cardiac troponin I (cTn-I); N/A; Human cardiac troponin I (cTn-I) ELISA Kit; cTn-I elisa kit
Reactivity
Human
Sequence Length
189
Samples
Human serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
2000 pg/mL-31.2 pg/mL
Sensitivity
Up to 12 pg/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for cTn-I elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human cTn- I monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cardiac troponin I
NCBI Official Symbol
LOC100462680
NCBI Protein Information
cardiac troponin I
