Human Deoxyribonuclease I ELISA Kit | Dnase I elisa kit

Human Deoxyribonuclease I ELISA Kit

Cat. Num. AAA16496

• Gene Names: DNASE1; DNL1; DRNI
• Reactivity: Human
• Synonyms: Deoxyribonuclease I; N/A; Human Deoxyribonuclease I ELISA Kit; Dnase I elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DNase I. No significant cross-reactivity or interference between DNase I and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DNase I and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Dnase I elisa kit

Principle of the Assay: DNase I ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DNase I antibody and an DNase I-HRP conjugate. The assay sample and buffer are incubated together with DNase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DNase I concentration since DNase I from samples and DNase I-HRP conjugate compete for the anti-DNase I antibody binding site. Since the number of sites is limited, as more sites are occupied by DNase I from the sample, fewer sites are left to bind DNase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DNase I concentration in each sample is interpolated from this standard curve.

Intended Uses: This DNase I ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DNase I. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for Dnase I elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 21361254
• NCBI GeneID: 1773
• NCBI Accession #: NP_005214.2
• NCBI GenBank Nucleotide #: NM_005223.3
• Molecular Weight: 31,434 Da
• NCBI Official Full Name: deoxyribonuclease-1
• NCBI Official Synonym Full Names: deoxyribonuclease I
• NCBI Official Symbol: DNASE1
• NCBI Official Synonym Symbols: DNL1; DRNI
• NCBI Protein Information: deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I
• UniProt Protein Name: Deoxyribonuclease-1
• UniProt Gene Name: DNASE1
• UniProt Synonym Gene Names: DNL1; DRNI; DNase I
• UniProt Entry Name: DNAS1_HUMAN

Frequently Asked Questions

How to test for DNase activity?

DNase activity is tested using ELISA kits designed to detect DNase enzyme. The assay involves incubating DNase with substrate DNA, measuring enzyme-catalyzed DNA degradation via colorimetric detection. Positive and negative controls verify assay performance. Results quantify DNase concentration or activity in samples.​

What is DNase used for?

DNase (deoxyribonuclease) is used in molecular biology for removing contaminating DNA from RNA preparations, digesting extracellular DNA in samples, treating DNA in biopharmaceutical manufacturing, and therapeutic applications. It's essential in research, diagnostics, and biopharmaceutical quality control processes.​

Is DNase active at room temperature?

DNase exhibits significant activity at room temperature (20-25°C) but reaches optimal activity at 37°C. Room temperature activity allows gentle sample handling in some applications. Enzyme activity increases substantially at physiological temperature, making 37°C the standard for activity assays and therapeutic applications.​

What does the DNase Activity ELISA Kit measure?

DNase ELISA kits measure the concentration or specific activity of DNase enzyme in samples through quantitative, colorimetric detection. The assay evaluates residual DNase contamination, confirms enzyme potency, and screens for DNase activity in pharmaceutical formulations and research materials.​

What is the detection sensitivity of the assay?

DNase ELISA kits typically detect concentrations from 0.1-1 ng/mL up to several μg/mL depending on kit formulation. Sensitivity enables the detection of trace DNase contamination in pharmaceutical products. AAA Biotech's kits provide sensitivity specifications suitable for quality control and research applications.​

How long does the DNase ELISA protocol take?

Complete DNase ELISA protocols typically require 2-4 hours total time: sample preparation (15-30 minutes), incubation steps (60-120 minutes), substrate reaction (10-20 minutes), and absorbance reading (5-10 minutes). Express protocols reduce total assay time to 60-90 minutes for rapid screening.​

What storage conditions are required for the kit?

DNase ELISA kit components are stored at 2-8°C in sealed, original packaging. Most kits remain stable for 12-18 months under proper refrigeration. Some specialized components may require -20°C storage. Verify precise storage requirements on product packaging to maintain assay reliability and component integrity.​