Mouse Deoxyribonuclease I ELISA Kit | Dnase I elisa kit

Mouse Deoxyribonuclease I ELISA Kit

Cat. Num. AAA16279

• Gene Names: DNASE1; DNL1; DRNI
• Reactivity: Mouse
• Synonyms: Deoxyribonuclease I; N/A; Mouse Deoxyribonuclease I ELISA Kit; Dnase I elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DNAI. No significant cross-reactivity or interference between DNAI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DNAI and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Dnase I elisa kit

Principle of the Assay: DNAI ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DNAI antibody and an DNAI-HRP conjugate. The assay sample and buffer are incubated together with DNAI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DNAI concentration since DNAI from samples and DNAI-HRP conjugate compete for the anti-DNAI antibody binding site. Since the number of sites is limited, as more sites are occupied by DNAI from the sample, fewer sites are left to bind DNAI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DNAI concentration in each sample is interpolated from this standard curve.

Intended Uses: This DNAI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DNAI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for Dnase I elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 21361254
• NCBI GeneID: 1773
• NCBI Accession #: NP_005214.2
• NCBI GenBank Nucleotide #: NM_005223.3
• Molecular Weight: 31,434 Da
• NCBI Official Full Name: deoxyribonuclease-1
• NCBI Official Synonym Full Names: deoxyribonuclease I
• NCBI Official Symbol: DNASE1
• NCBI Official Synonym Symbols: DNL1; DRNI
• NCBI Protein Information: deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I
• UniProt Protein Name: Deoxyribonuclease-1
• UniProt Gene Name: DNASE1
• UniProt Synonym Gene Names: DNL1; DRNI; DNase I
• UniProt Entry Name: DNAS1_HUMAN