Mouse Osteocalcin ELISA Kit | Bglap elisa kit
Mouse Osteocalcin ELISA Kit
Gene Names
Bglap; OC; BGP; OG1; mOC-A; Bglap1
Reactivity
Mouse
Synonyms
Osteocalcin; N/A; Mouse Osteocalcin ELISA Kit; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; Bglap; Bglap elisa kit
Reactivity
Mouse
Specificity
Recombinant and natural Mouse VEGF-C
Sequence Length
95
Samples
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
Detection Range
15.6-1000 pg/mL
Intra-assay Precision
Intra-Assay CV: <=6.1%
Inter-assay Precision
Inter-Assay CV: <=9.9%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Related Product Information for Bglap elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,549 Da
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma carboxyglutamate protein
NCBI Official Symbol
Bglap
NCBI Official Synonym Symbols
OC; BGP; OG1; mOC-A; Bglap1
NCBI Protein Information
osteocalcin
UniProt Protein Name
Osteocalcin
UniProt Gene Name
Bglap
UniProt Synonym Gene Names
BGP
UniProt Entry Name
OSTCN_MOUSE