Mouse Osteocalcin (OC) ELISA Kit | OC elisa kit
Mouse Osteocalcin (OC) ELISA Kit
Gene Names
Bglap; OC; BGP; OG1; mOC-A; Bglap1
Reactivity
Mouse
Synonyms
Osteocalcin (OC); N/A; Mouse Osteocalcin (OC) ELISA Kit; BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein; OC elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of OC. No significant cross-reactivity or interference between OC and analogues was observed.
Sequence Length
95
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
< 23.58pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Standard Curve (Sample)
Related Product Information for OC elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of OC in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,549 Da
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma carboxyglutamate protein
NCBI Official Symbol
Bglap
NCBI Official Synonym Symbols
OC; BGP; OG1; mOC-A; Bglap1
NCBI Protein Information
osteocalcin
UniProt Protein Name
Osteocalcin
UniProt Gene Name
Bglap
UniProt Synonym Gene Names
BGP
