Chicken Osteocalcin (OC) ELISA Kit | OC elisa kit
Gallus Osteocalcin (OC) ELISA Kit
Gene Names
BGLAP; OC; BGP; OCN
Reactivity
Chicken
Synonyms
Osteocalcin (OC); N/A; Gallus Osteocalcin (OC) ELISA Kit; BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein; OC elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of osteocalcin. No significant cross-reactivity or interference between osteocalcin and analogues was observed.
Sequence Length
100
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.113ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level osteocalcin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level osteocalcin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for OC elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of osteocalcin in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to osteocalcin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to osteocalcin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain osteocalcin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of osteocalcin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to osteocalcin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to osteocalcin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain osteocalcin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of osteocalcin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,963 Da
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma-carboxyglutamate protein
NCBI Official Symbol
BGLAP
NCBI Official Synonym Symbols
OC; BGP; OCN
NCBI Protein Information
osteocalcin
UniProt Protein Name
Osteocalcin
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
