Mouse Actin Monoclonal Antibody | anti-ACTA1 antibody

Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA13822

• Gene Names: ACTA2; AAT6; ACTSA; MYMY5
• Reactivity: Human, Rabbit, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Actin, Antibody; Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody; ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1; anti-ACTA1 antibody

• Host: Mouse
• Reactivity: Human, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: MSA/953
• Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 377

• Applicable Applications for anti-ACTA1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-1.0ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Synthetic peptide common among human muscle actins
• Hu-Chromosome Location: 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
• Positive Control: Muscle or sarcoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistchemistry)

(Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

WB (Western Blot)

(Western Blot of hSKM Cell Lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

References

Barkalow, K. and Hartwig, J.H. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr. Biol. 5: 1000-1002.

NCBI and Uniprot Product Information

• NCBI GI #: 213688375
• NCBI GeneID: 59
• NCBI Accession #: NP_001135417.1
• NCBI GenBank Nucleotide #: NM_001141945.2
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• Molecular Weight: 42,009 Da
• NCBI Official Full Name: actin, aortic smooth muscle
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: AAT6; ACTSA; MYMY5
• NCBI Protein Information: actin, aortic smooth muscle
• UniProt Protein Name: Actin, aortic smooth muscle
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS
• UniProt Entry Name: ACTA_HUMAN