Mouse IL-2R alpha (CD25) Antibody | anti-IL-2Ralpha antibody

Anti-IL-2R alpha (CD25)[Basiliximab], Mouse IgG1

Cat. Num. AAA14188

• Gene Names: IL2RA; CD25; IL2R; TCGFR; IDDM10
• Reactivity: Human, Rhesus Monkey, Cynomolgus Monkey
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Protein A affinity purified
• Synonyms: IL-2R alpha (CD25); Anti-IL-2R alpha (CD25)[Basiliximab]; Mouse IgG1; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL-2-RA; IL2-RA; TAC antigen; p55; CD25.; anti-IL-2Ralpha antibody

• Host: Mouse
• Reactivity: Human, Rhesus Monkey, Cynomolgus Monkey
• Isotype: Mouse IgG1, Kappa
• Clone Number: Basiliximab
• Specificity: Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (alphabetagammac) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IL-2Ralpha antibody: Flow Cytometry (FC/FACS), Blocking (BL), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Immunogen: Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
• Chimeric Use Statement: This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

WB (Western Blot)

(Western Blot using anti-IL2R alpha (CD25) antibody AAA14188 Human thyroid cancer (lane 1), rat spleen (lane 2), mouse lymph node (lane 3), mouse thymus (lane 4) and rat small intestines (lane 5)samples were resolved on a 10% SDS PAGE gel and blots probed with AAA14188-10.0 at 1 ug/ml before being detected by a secondary antibody.)

FCM (Flow Cytometry)

(Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Basiliximab (AAA14188) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Basiliximab (AAA14188-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

IF (Immunofluorescence)

(Immunofluorescent staining of rat thymus using anti-CD25 antibody AAA14188 Formaldehyde-fixed rat thymus slices were stained with AAA14188-10.0 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. AAA14188 shows weak labelling of rat cells by IF.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat spleens using anti-CD25 antibody AAA14188 Formalin fixed rat spleen slices were were stained with a AAA14188-10.0 at 5 ug/ml. The anti-human CD25 antibody AAA14188 also shows some cross-reactivity with rat in IHC.)

FCM (Flow Cytometry)

(Flow-cytometry using anti-CD25 antibody Basiliximab (AAA14188) Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Basiliximab (AAA14188-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

FCM (Flow Cytometry)

(Flow-cytometry using anti-CD25 antibody Basiliximab (AAA14188) Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Basiliximab (AAA14188-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

Related Product Information for anti-IL-2Ralpha antibody

Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1kappa constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.

Product Categories/Family for anti-IL-2Ralpha antibody

biosimilar

References

Kovarik et al. Disposition of basiliximab, an interleukin-2 receptor monoclonal antibody, in recipients of mismatched cadaver renal allografts. Transplantation. 1997 Dec 27;64(12):1701-5. PMID:9422405

NCBI and Uniprot Product Information

• NCBI GI #: 4557667
• NCBI GeneID: 3559
• NCBI Accession #: NP_000408.1
• NCBI GenBank Nucleotide #: NM_000417.2
• UniProt Accession #: P01589; Q5W007
• Molecular Weight: 30,819 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: CD25; IL2R; TCGFR; IDDM10
• NCBI Protein Information: interleukin-2 receptor subunit alpha; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_HUMAN