Human secreted frizzled-related protein 5 ELISA Kit | SFRP5 elisa kit
Human secreted frizzled-related protein 5, SFRP5 ELISA Kit
Gene Names
SFRP5; SARP3
Reactivity
Human
Synonyms
secreted frizzled-related protein 5; N/A; Human secreted frizzled-related protein 5, SFRP5 ELISA Kit; Human secreted frizzled-related protein 5 (SFRP5) ELISA Kit; SARP3; secreted apoptosis related protein 3; SFRP5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SERPINB7. No significant cross-reactivity or interference between human SERPINB7 and analogues was observed.
Sequence Length
317
Samples
Serum, Plasma, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
12.5 pg/ml-800 pg/ml
Sensitivity
3.125 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for SFRP5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,563 Da
NCBI Official Full Name
secreted frizzled-related protein 5
NCBI Official Synonym Full Names
secreted frizzled-related protein 5
NCBI Official Symbol
SFRP5
NCBI Official Synonym Symbols
SARP3
NCBI Protein Information
secreted frizzled-related protein 5; FRP-1b; SARP-3; sFRP-5; frizzled-related protein 1b; secreted apoptosis related protein 3; secreted apoptosis-related protein 3
UniProt Protein Name
Secreted frizzled-related protein 5
UniProt Gene Name
SFRP5
UniProt Synonym Gene Names
FRP1B; SARP3; sFRP-5; FRP-1b; SARP-3
UniProt Entry Name
SFRP5_HUMAN