Human hypoxia-inducible factor 1alpha (HIF-1alpha) ELISA Kit | HIF-1alpha elisa kit

Human hypoxia-inducible factor 1alpha (HIF-1alpha) ELISA Kit

Cat. Num. AAA15430

• Gene Names: HIF1A; HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
• Reactivity: Human
• Synonyms: hypoxia-inducible factor 1alpha (HIF-1alpha); Human hypoxia-inducible factor 1alpha (HIF-1alpha) ELISA Kit; hypoxia-inducible factor 1a (HIF-1a) ; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein|hypoxia-inducible factor 1 alpha isoform I.3|hypoxia-inducible factor 1; alpha subunit|hypoxi; HIF-1alpha elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human HGFA. No significant cross-reactivity or interference between human HGFA and analogues was observed.
• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: 19.5 pg/ml.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for HIF-1alpha elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGFA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGFA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504385
• NCBI GeneID: 3091
• NCBI Accession #: NP_001521.1
• NCBI GenBank Nucleotide #: NM_001530.3
• UniProt Accession #: Q16665; Q53XP6; Q96PT9; Q9UPB1
• Molecular Weight: 92,670 Da
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
• NCBI Protein Information: hypoxia-inducible factor 1-alpha; HIF-1-alpha; OTTHUMP00000179061; OTTHUMP00000179062; OTTHUMP00000179063; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: BHLHE78; MOP1; PASD8
• UniProt Entry Name: HIF1A_HUMAN