Standard Curve (Sample)
Mouse hypoxia-inducible factor 1alpha ELISA Kit | HIF-1alpha elisa kit
Mouse hypoxia-inducible factor 1alpha ELISA Kit
Gene Names
HIF1A; HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Mouse
Synonyms
hypoxia-inducible factor 1alpha; Mouse hypoxia-inducible factor 1alpha ELISA Kit; hypoxia-inducible factor 1a; hypoxia-inducible factor 1alpha (Mouse); HIF-1alpha elisa kit
Reactivity
Mouse
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HIF-1alpha elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma
Intended Uses: This HIF1a ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of HIF1a in the sample, this HIF1a ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus HIF1a concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of HIF1a utilizes a polyclonal anti-HIF1a antibody and an HIF1a-HRP conjugate. The assay sample and buffer are incubated together with HIF1a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HIF1a concentration since HIF1a from samples and HIF1a-HRP conjugate compete for the anti-HIF1a antibody binding site. Since the number of sites is limited, as more sites are occupied by HIF1a from the sample, fewer sites are left to bind HIF1a-HRP conjugate. Standards of known HIF1a concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of HIF1a. The HIF1a concentration in each sample is interpolated from this standard curve.
Intended Uses: This HIF1a ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of HIF1a in the sample, this HIF1a ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus HIF1a concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of HIF1a utilizes a polyclonal anti-HIF1a antibody and an HIF1a-HRP conjugate. The assay sample and buffer are incubated together with HIF1a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HIF1a concentration since HIF1a from samples and HIF1a-HRP conjugate compete for the anti-HIF1a antibody binding site. Since the number of sites is limited, as more sites are occupied by HIF1a from the sample, fewer sites are left to bind HIF1a-HRP conjugate. Standards of known HIF1a concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of HIF1a. The HIF1a concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HIF-1alpha elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,670 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
NCBI Protein Information
hypoxia-inducible factor 1-alpha; HIF-1-alpha; OTTHUMP00000179061; OTTHUMP00000179062; OTTHUMP00000179063; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The Mouse HIF-1alpha hif1a (Catalog #AAA16167) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16167 ELISA Kit recognizes Mouse HIF-1alpha. It is sometimes possible for the material contained within the vial of "hypoxia-inducible factor 1alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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