Human fibroblast growth factor 23 ELISA Kit | FGF23 elisa kit
Human fibroblast growth factor-23, FGF-23 ELISA Kit
Gene Names
FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
Reactivity
Human
Synonyms
fibroblast growth factor 23; N/A; Human fibroblast growth factor-23, FGF-23 ELISA Kit; Human fibroblast growth factor-23; FGF-23 ELISA Kit; ADHR; HPDR2; HYPF; PHPTC; tumor-derived hypophosphatemia inducing factor; FGF23 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human FGF21. No significant cross-reactivity or interference between human FGF21 and analogues was observed.
Sequence Length
251
Samples
Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for FGF23 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF21 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,954 Da
NCBI Official Full Name
fibroblast growth factor 23
NCBI Official Synonym Full Names
fibroblast growth factor 23
NCBI Official Symbol
FGF23
NCBI Official Synonym Symbols
ADHR; FGFN; HYPF; HPDR2; PHPTC
NCBI Protein Information
fibroblast growth factor 23; phosphatonin; tumor-derived hypophosphatemia inducing factor
UniProt Protein Name
Fibroblast growth factor 23
UniProt Gene Name
FGF23
UniProt Synonym Gene Names
HYPF; UNQ3027/PRO9828; FGF-23
UniProt Entry Name
FGF23_HUMAN