Guinea Pig Immunoglobulin E ELISA Kit | IgE elisa kit
Guinea pig Immunoglobulin E ELISA Kit
Reactivity
Guinea Pig
Synonyms
Immunoglobulin E; N/A; Guinea pig Immunoglobulin E ELISA Kit; IgE elisa kit
Reactivity
Guinea Pig
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This IgE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IgE. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for IgE elisa kit
Principle of the Assay: IgE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IgE antibody and an IgE-HRP conjugate. The assay sample and buffer are incubated together with IgE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgE concentration since IgE from samples and IgE-HRP conjugate compete for the anti-IgE antibody binding site. Since the number of sites is limited, as more sites are occupied by IgE from the sample, fewer sites are left to bind IgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgE concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IgE elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
immunoglobulin E heavy chain, partial
