Human leukocyte antigen E (HLA-E) ELISA Kit | HLA-E elisa kit
Human leukocyte antigen E (HLA-E) ELISA Kit
Gene Names
HLA-F; HLAF; CDA12; HLA-5.4; HLA-CDA12
Reactivity
Human
Synonyms
leukocyte antigen E (HLA-E); N/A; Human leukocyte antigen E (HLA-E) ELISA Kit; HLA-E elisa kit
Reactivity
Human
Specificity
No cross-reaction with other factors.
Sequence Length
362
Assay Type
Sandwich
Detection Range
200 U/mL-3.12 U/mL
Sensitivity
Up to 0.6 U/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for HLA-E elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human HLA-E monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,438 Da
NCBI Official Full Name
leukocyte antigen F
NCBI Official Synonym Full Names
major histocompatibility complex, class I, F
NCBI Official Symbol
HLA-F
NCBI Official Synonym Symbols
HLAF; CDA12; HLA-5.4; HLA-CDA12
NCBI Protein Information
HLA class I histocompatibility antigen, alpha chain F; HLA F antigen; leukocyte antigen F; HLA class I molecule; MHC class Ib antigen; MHC class I antigen F
UniProt Protein Name
HLA class I histocompatibility antigen, alpha chain F
UniProt Gene Name
HLA-F
UniProt Synonym Gene Names
HLA-5.4; HLAF
UniProt Entry Name
HLAF_HUMAN