Mouse Mast cell tryptase ELISA Kit | MCT elisa kit
Mouse Mast cell tryptase ELISA Kit
Reactivity
Mouse
Synonyms
Mast cell tryptase; N/A; Mouse Mast cell tryptase ELISA Kit; MCT elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of MCT. No significant cross-reactivity or interference between MCT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MCT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for MCT elisa kit
Intended Uses: This MCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MCT. This ELISA kit for research use only!
Principle of the Assay: MCT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MCT. Standards or samples are then added to the microtiter plate wells and MCT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MCT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MCT are added to each well to "sandwich" the MCT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MCT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MCT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MCT. Standards or samples are then added to the microtiter plate wells and MCT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MCT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MCT are added to each well to "sandwich" the MCT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MCT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MCT elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
54,994 Da
NCBI Official Full Name
MCT
UniProt Protein Name
Monocarboxylate transporter 6
UniProt Gene Name
SLC16A5
UniProt Synonym Gene Names
MCT5; MCT6; MCT 6; MCT 5
UniProt Entry Name
MOT6_HUMAN
