Rat tryptase alpha/beta 1 ELISA Kit | TPSAB1 elisa kit
Rat Mast Cell Tryptase, MCT ELISA Kit
Gene Names
Tpsab1; Mcpt7; TPSB1; rMCP-7
Reactivity
Rat
Synonyms
tryptase alpha/beta 1; N/A; Rat Mast Cell Tryptase, MCT ELISA Kit; Rat Mast Cell Tryptase; MCT ELISA Kit; TPS1; TPS2; TPSB1; mast cell alpha II tryptase; mast cell beta I tryptase; tryptase alpha II; tryptase beta 1; tryptase beta I; tryptase-I; tryptase-III; TPSAB1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat MCT. No significant cross-reactivity or interference between rat MCT and analogues was observed.
Sequence Length
310
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, urine
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
< 0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for TPSAB1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,400 Da
NCBI Official Full Name
tryptase
NCBI Official Synonym Full Names
tryptase alpha/beta 1
NCBI Official Symbol
Tpsab1
NCBI Official Synonym Symbols
Mcpt7; TPSB1; rMCP-7
NCBI Protein Information
tryptase; MMCP-7; tryptase, skin; tryptase beta 1; mast cell protease 7; tryptase alpha/beta-1
UniProt Protein Name
Tryptase
UniProt Gene Name
Tpsab1
UniProt Synonym Gene Names
Mcp7; Mcpt7; Tpsb1; rMCP-7
UniProt Entry Name
TRYB1_RAT