Rat alpha Synuclein ELISA Kit | alpha-SYN elisa kit

Rat alpha Synuclein ELISA Kit

Cat. Num. AAA16124

• Gene Names: Snca; NACP; alphaSYN
• Reactivity: Rat
• Synonyms: alpha Synuclein; N/A; Rat alpha Synuclein ELISA Kit; Rat a Synuclein ELISA Kit; alpha-SYN elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of SNCalpha. No significant cross-reactivity or interference between SNCalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SNCalpha and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for alpha-SYN elisa kit

Principle of the Assay: SNCalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SNCalpha antibody and an SNCalpha-HRP conjugate. The assay sample and buffer are incubated together with SNCalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNCalpha concentration since SNCalpha from samples and SNCalpha-HRP conjugate compete for the anti-SNCalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by SNCalpha from the sample, fewer sites are left to bind SNCalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNCalpha concentration in each sample is interpolated from this standard curve.

Intended Uses: This SNCalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SNCalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for alpha-SYN elisa kit

Neurobiology

References

Increase of Oxidative Stres ?-Synuclein in Wistar Rat's. Arief Budi Yulianti, Sony Heru Sumarsono, Ahmad Ridwan & Ayda T. Yusuf (2012)

NCBI and Uniprot Product Information

• NCBI GI #: 109638747
• NCBI GeneID: 20617
• NCBI Accession #: NP_001035916.1
• NCBI GenBank Nucleotide #: NM_001042451.2
• Molecular Weight: 12,345 Da
• NCBI Official Full Name: alpha-synuclein
• NCBI Official Synonym Full Names: synuclein, alpha
• NCBI Official Symbol: Snca
• NCBI Official Synonym Symbols: NACP; alphaSYN
• NCBI Protein Information: alpha-synuclein; non-A4 component of amyloid; non-A beta component of AD amyloid
• UniProt Protein Name: Alpha-synuclein
• UniProt Gene Name: Snca
• UniProt Synonym Gene Names: Syn; NACP
• UniProt Entry Name: SYUA_MOUSE