Mouse Apolipoprotein C3 ELISA Kit | APOC3 elisa kit
Mouse Apolipoprotein C3 ELISA Kit
Gene Names
APOC3; HALP2; APOCIII
Reactivity
Mouse
Synonyms
Apolipoprotein C3; N/A; Mouse Apolipoprotein C3 ELISA Kit; APOC3 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for APOC3 elisa kit
Intended Uses: This ApoC3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ApoC3. This ELISA kit for research use only!
Principle of the Assay: ApoC3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoC3. Standards or samples are then added to the microtiter plate wells and ApoC3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoC3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoC3 are added to each well to "sandwich" the ApoC3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoC3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoC3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ApoC3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoC3. Standards or samples are then added to the microtiter plate wells and ApoC3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoC3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoC3 are added to each well to "sandwich" the ApoC3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoC3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoC3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for APOC3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,852 Da
NCBI Official Full Name
apolipoprotein C-III
NCBI Official Synonym Full Names
apolipoprotein C-III
NCBI Official Symbol
APOC3
NCBI Official Synonym Symbols
HALP2; APOCIII
NCBI Protein Information
apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3
UniProt Protein Name
Apolipoprotein C-III
UniProt Gene Name
APOC3
UniProt Synonym Gene Names
Apo-CIII; ApoC-III
UniProt Entry Name
APOC3_HUMAN
