Human Antioncogene p21 protein ELISA Kit | p21 elisa kit

Human Antioncogene p21 protein ELISA Kit

Cat. Num. AAA16589

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Human
• Synonyms: Antioncogene p21 protein; Human Antioncogene p21 protein ELISA Kit; p21 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of P21. No significant cross-reactivity or interference between P21 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between P21 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for p21 elisa kit

Principle of the Assay: P21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-P21 antibody and an P21-HRP conjugate. The assay sample and buffer are incubated together with P21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P21 concentration since P21 from samples and P21-HRP conjugate compete for the anti-P21 antibody binding site. Since the number of sites is limited, as more sites are occupied by P21 from the sample, fewer sites are left to bind P21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P21 concentration in each sample is interpolated from this standard curve.

Intended Uses: This P21 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human P21. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for p21 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 453135
• NCBI GeneID: 1026
• Molecular Weight: 18,119 Da
• NCBI Official Full Name: p21
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1; DNA synthesis inhibitor; CDK-interacting protein 1; CDK-interaction protein 1; wild-type p53-activated fragment 1; melanoma differentiation associated protein 6
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
• UniProt Entry Name: CDN1A_HUMAN