Canine Cadherin 5 ELISA Kit | CDH5 elisa kit
Canine Cadherin 5 ELISA Kit
Gene Names
CDH5; 7B4; CD144
Reactivity
Canine
Synonyms
Cadherin 5; N/A; Canine Cadherin 5 ELISA Kit; CDH5 elisa kit
Reactivity
Canine
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CDH5 elisa kit
Intended Uses: This CDC5L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCDC5L. This ELISA kit for research use only!
Principle of the Assay: CDC5L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC5L antibody and an CDC5L-HRP conjugate. The assay sample and buffer are incubated together with CDC5L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC5L concentration since CDC5L from samples and CDC5L-HRP conjugate compete for the anti-CDC5L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC5L from the sample, fewer sites are left to bind CDC5L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC5L concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CDC5L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC5L antibody and an CDC5L-HRP conjugate. The assay sample and buffer are incubated together with CDC5L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC5L concentration since CDC5L from samples and CDC5L-HRP conjugate compete for the anti-CDC5L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC5L from the sample, fewer sites are left to bind CDC5L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC5L concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CDH5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,499 Da
NCBI Official Full Name
cadherin-5 preproprotein
NCBI Official Synonym Full Names
cadherin 5, type 2 (vascular endothelium)
NCBI Official Symbol
CDH5
NCBI Official Synonym Symbols
7B4; CD144
NCBI Protein Information
cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium)
UniProt Protein Name
Cadherin-5
UniProt Gene Name
CDH5
UniProt Synonym Gene Names
VE-cadherin
UniProt Entry Name
CADH5_HUMAN
