Canine Visfatin ELISA Kit | VF elisa kit

Canine Visfatin ELISA Kit

Cat. Num. AAA16739

• Reactivity: Canine
• Synonyms: Visfatin; N/A; Canine Visfatin ELISA Kit; VF elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of VF. No significant cross-reactivity or interference between VF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VF and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for VF elisa kit

Principle of the Assay: VF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VF antibody and an VF-HRP conjugate. The assay sample and buffer are incubated together with VF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VF concentration since VF from samples and VF-HRP conjugate compete for the anti-VF antibody binding site. Since the number of sites is limited, as more sites are occupied by VF from the sample, fewer sites are left to bind VF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VF concentration in each sample is interpolated from this standard curve.

Intended Uses: This VF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine VF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for VF elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 160415772
• Molecular Weight: 55,951 Da
• NCBI Official Full Name: visfatin
• UniProt Protein Name: Visfatin
• UniProt Gene Name: PBEF
• UniProt Entry Name: A9LRS6_9TELE