Bovine Heat Shock Protein 70 ELISA Kit | HSP70 elisa kit

Bovine Heat Shock Protein 70 ELISA Kit

Cat. Num. AAA16802

• Reactivity: Bovine
• Synonyms: Heat Shock Protein 70; N/A; Bovine Heat Shock Protein 70 ELISA Kit; HSP70 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSP70. No significant cross-reactivity or interference between HSP70 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP70 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for HSP70 elisa kit

Principle of the Assay: HSP70 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HSP70 antibody and an HSP70-HRP conjugate. The assay sample and buffer are incubated together with HSP70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP70 concentration since HSP70 from samples and HSP70-HRP conjugate compete for the anti-HSP70 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP70 from the sample, fewer sites are left to bind HSP70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve.

Intended Uses: This HSP70 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HSP70. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for HSP70 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 476003
• Molecular Weight: 67,016 Da
• NCBI Official Full Name: HSP70
• UniProt Protein Name: HSP70
• UniProt Entry Name: Q40058_HORVU