Reactivity
Monkey
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for F1+2 elisa kit
Intended Uses: This PEPCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PEPCK. This ELISA kit for research use only!
Principle of the Assay: PEPCK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PEPCK antibody and an PEPCK-HRP conjugate. The assay sample and buffer are incubated together with PEPCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PEPCK concentration since PEPCK from samples and PEPCK-HRP conjugate compete for the anti-PEPCK antibody binding site. Since the number of sites is limited, as more sites are occupied by PEPCK from the sample, fewer sites are left to bind PEPCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PEPCK concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PEPCK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PEPCK antibody and an PEPCK-HRP conjugate. The assay sample and buffer are incubated together with PEPCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PEPCK concentration since PEPCK from samples and PEPCK-HRP conjugate compete for the anti-PEPCK antibody binding site. Since the number of sites is limited, as more sites are occupied by PEPCK from the sample, fewer sites are left to bind PEPCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PEPCK concentration in each sample is interpolated from this standard curve.
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