Reactivity                
                
                    Human                
            
                    Specificity                
                
                    No cross-reaction with other factors.                
            
                    Sequence Length                
                
                    622                
            
                        Assay Type                    
                    
                        Sandwich                    
                
                        Detection Range                    
                    
                        100 ng/mL-1.56 ng/mL                    
                
                        Sensitivity                    
                    
                        Up to 0.5 ng/mL                    
                
                        Intra-assay Precision                    
                    
                        <= 8%                    
                
                        Inter-assay Precision                    
                    
                        <= 12%                    
                
                    Preparation and Storage                
                
                    Store all reagents at 2-8 degree C.                
            
                    Related Product Information for F1+2 elisa kit                
                
                 
                    Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human F1+2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.                 
                
            NCBI and Uniprot Product Information
                    NCBI GI #                
                
            
                    NCBI GeneID                
                
            
                    NCBI Accession #                
                
            
                    NCBI GenBank Nucleotide #                
                
            
                    Molecular Weight                
                
                                            70,037 Da                                    
            
                    NCBI Official Full Name                
                
                    prothrombin preproprotein                
            
                    NCBI Official Synonym Full Names                
                
                    coagulation factor II (thrombin)                
            
                    NCBI Official Symbol                
                
                    F2                 
            
                    NCBI Official Synonym Symbols                
                
                    PT; THPH1; RPRGL2                 
            
                    NCBI Protein Information                
                
                    prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II                
            
                    UniProt Protein Name                
                
                    Prothrombin                
            
                    UniProt Gene Name                
                
                    F2                
            
                    UniProt Entry Name                
                
                    THRB_HUMAN                
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Product Notes
The Human F1+2 f2 (Catalog #AAA12531) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA12531 ELISA Kit recognizes Human F1+2. It is sometimes possible for the material contained within the vial of "Prothrombin Fragment 1+2 (F1+2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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