Human prothrombin fragment 1+2, F1+2 ELISA Kit | F1+2 elisa kit
Human prothrombin fragment 1+2, F1+2 ELISA Kit
Reactivity
Human
Synonyms
prothrombin fragment 1+2, F1+2; N/A; Human prothrombin fragment 1+2, F1+2 ELISA Kit; Human prothrombin fragment 1+2; F1+2 ELISA kit; prothrombin fragment 1+2; F1+2; F1+2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human F1+2. No significant cross-reactivity or interference between human F1+2 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
0.03 nmol/L-2 nmol/L
Sensitivity
< 0.007 nmol/L
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for F1+2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F1+2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F1+2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F1+2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F1+2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
