Porcine Neuron Specific Enolase ELISA Kit | NSE elisa kit
Porcine Neuron Specific Enolase ELISA Kit
Reactivity
Porcine
Synonyms
Neuron Specific Enolase; N/A; Porcine Neuron Specific Enolase ELISA Kit; NSE elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of NSE. No significant cross-reactivity or interference between NSE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NSE and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for NSE elisa kit
Intended Uses: This NSE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine NSE. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: NSE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NSE antibody and an NSE-HRP conjugate. The assay sample and buffer are incubated together with NSE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NSE concentration since NSE from samples and NSE-HRP conjugate compete for the anti-NSE antibody binding site. Since the number of sites is limited, as more sites are occupied by NSE from the sample, fewer sites are left to bind NSE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NSE concentration in each sample is interpolated from this standard curve.
Principle of the Assay: NSE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NSE antibody and an NSE-HRP conjugate. The assay sample and buffer are incubated together with NSE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NSE concentration since NSE from samples and NSE-HRP conjugate compete for the anti-NSE antibody binding site. Since the number of sites is limited, as more sites are occupied by NSE from the sample, fewer sites are left to bind NSE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NSE concentration in each sample is interpolated from this standard curve.
Product Categories/Family for NSE elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
47,307 Da
NCBI Official Full Name
neuron-specific enolase
UniProt Protein Name
Neuron-specific enolase
UniProt Gene Name
NSE
UniProt Entry Name
H9BVF0_GECJA
