Human Pancreatic prohormone ELISA Kit | PPY elisa kit
Human Pancreatic prohormone ELISA Kit
Gene Names
PPY; PP; PNP
Reactivity
Human
Synonyms
Pancreatic prohormone; N/A; Human Pancreatic prohormone ELISA Kit; PPY/INN/Obinepitide/PNP/PP/Obinepitide/pancreatic polypeptide (ppy)/pancreatic polypeptide/pancreatic polypeptide Y/pancreatic prohormone; PPY elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PGF2alpha. No significant cross-reactivity or interference between PGF2alpha and analogues is observed.
Sequence Length
95
Assay Type
Competitive ELISA, Coated with Antigen
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
46.875-3000pg/ml
Sensitivity
28.125pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGF2alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGF2alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for PPY elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with PGF2alpha. During the reaction, PGF2alpha in the sample or standard competes with a fixed amount of PGF2alpha on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGF2alpha in the samples is then determined by comparing the O D of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,786 Da
NCBI Official Full Name
pancreatic prohormone isoform 1 preproprotein
NCBI Official Synonym Full Names
pancreatic polypeptide
NCBI Official Symbol
PPY
NCBI Official Synonym Symbols
PP; PNP
NCBI Protein Information
pancreatic prohormone
UniProt Protein Name
Pancreatic prohormone
UniProt Gene Name
PPY
UniProt Synonym Gene Names
PNP; PP; PH; PI
UniProt Entry Name
PAHO_HUMAN
