Human Regulatory-associated protein of mTOR ELISA Kit | RPTOR elisa kit

Human Regulatory-associated protein of mTOR ELISA Kit

Cat. Num. AAA17537

• Gene Names: Rptor; Rap; Raptor; mKIAA1303; 4932417H02Rik
• Reactivity: Human
• Synonyms: Regulatory-associated protein of mTOR; N/A; Human Regulatory-associated protein of mTOR ELISA Kit; RPTOR (Regulatory-associated protein of mTOR)/KIAA1303/Raptor/RAPTOR/p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats/KOG1/Mip1/p150 target of rapamycin (TOR)-scaffold protein/regulatory associated protein of MTOR; complex 1; RPTOR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RPTOR. No significant cross-reactivity or interference between RPTOR and analogues was observed.
• Sequence Length: 1335
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RPTOR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RPTOR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for RPTOR elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RPTOR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RPTOR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RPTOR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RPTOR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 154146249
• NCBI GeneID: 74370
• NCBI Accession #: NP_083174.2
• NCBI GenBank Nucleotide #: NM_028898.3
• Molecular Weight: 33,651 Da
• NCBI Official Full Name: regulatory-associated protein of mTOR isoform 1
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: Rptor
• NCBI Official Synonym Symbols: Rap; Raptor; mKIAA1303; 4932417H02Rik
• NCBI Protein Information: regulatory-associated protein of mTOR
• UniProt Protein Name: Regulatory-associated protein of mTOR
• UniProt Gene Name: Rptor
• UniProt Synonym Gene Names: Raptor; Raptor
• UniProt Entry Name: RPTOR_MOUSE