Human mammalian target of rapamycin (mTOR) ELISA Kit | mTOR elisa kit
Human mammalian target of rapamycin (mTOR) ELISA Kit
Reactivity
Human
Synonyms
mammalian target of rapamycin (mTOR); N/A; Human mammalian target of rapamycin (mTOR) ELISA Kit; mTOR elisa kit
Reactivity
Human
Sequence Length
2549
Assay Type
Sandwich
Detection Range
50 ng/mL-0.78 ng/mL
Sensitivity
Up to 0.1 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for mTOR elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human mTOR monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
288,647 Da
NCBI Official Full Name
mammalian target of rapamycin
NCBI Official Symbol
MTOR
NCBI Protein Information
mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin (serine/threonine kinase); mammalian target of rapamycin
UniProt Protein Name
Mammalian target of rapamycin
UniProt Gene Name
mTOR
UniProt Entry Name
D9I5T4_CAPHI
