Mouse IL-1 beta ELISA Kit | IL1B elisa kit

Mouse IL-1 beta ELISA Kit

Cat. Num. AAA17899

• Gene Names: Il1b; Il-1b; IL-1beta
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: IL-1 beta; Mouse IL-1 beta ELISA Kit; IL1F2; Interleukin-1 beta; Catabolin; IL1B elisa kit

• Reactivity: Mouse
• Specificity: The Mouse IL-1 beta ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-1 beta proteins within the range of 31.2 pg/ml - 2,000 pg/ml.
• Sequence Length: 269
• Samples: Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Sensitivity: < 15 pg/ml

• Applicable Applications for IL1B elisa kit: Sandwich ELISA (SE)
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for IL1B elisa kit

Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Principle of the Assay: The Mouse IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1 beta in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 6680415
• NCBI GeneID: 16176
• NCBI Accession #: NP_032387.1
• NCBI GenBank Nucleotide #: NM_008361.3
• UniProt Accession #: P10749; Q2M4J6
• Molecular Weight: 30,931 Da
• NCBI Official Full Name: interleukin-1 beta
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: Il1b
• NCBI Official Synonym Symbols: Il-1b; IL-1beta
• NCBI Protein Information: interleukin-1 beta; IL-1 beta
• UniProt Protein Name: Interleukin-1 beta
• UniProt Gene Name: Il1b
• UniProt Synonym Gene Names: IL-1 beta
• UniProt Entry Name: IL1B_MOUSE