Rabbit TRIM6 Polyclonal Antibody | anti-TRIM6 antibody

Anti-TRIM6 Antibody

Cat. Num. AAA19195

• Gene Names: TRIM6; RNF89
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: TRIM6, Antibody; Anti-TRIM6 Antibody; Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89; anti-TRIM6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 313

• Applicable Applications for anti-TRIM6 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC), Direct ELISA (EIA)
• Application Notes: WB: Concentration :0.25-0.5ug/ml; Tested Species: Human, Mouse, Rat; ; IHC-P (Embedded Section): Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; ; ICC: Concentration: 2ug/ml; Tested Species: Human; ; IF: Concentration: 2ug/ml; Tested Species: Human; ; FC: Concentration: 1-3ug/1x10⁶ cells; Tested Species: Human; ; Direct ELISA: Concentration: 0.1-0.5ug/ml; Tested Species: Human; ; Tested Species: In-house tested species with positive results.; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: E Coli-derived human TRIM6 recombinant protein (Position: Q50-H349).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Relevant Detection Systems: QC Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG ( ) for Western Blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay ( ) for Immunohistochemistry IHC(P) and Immunocytochemistry (ICC)
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

WB (Western Blot)

(Figure 1. Western blot analysis of TRIM6 using anti-TRIM6 antibody.)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

FCM (Flow Cytometry)

Related Product Information for anti-TRIM6 antibody

Description: Rabbit IgG polyclonal antibody for TRIM6 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.

Background: Tripartite motif-containing protein 6 is a protein that in humans is encoded by the TRIM6 gene. This gene is mapped to 11p15.4. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, B-box type 1 and B-box type 2 domain, and a coiled-coil region. The protein localizes to the nucleus, but its specific function has not been identified. This gene is mapped to chromosome 11p15, where it resides within a TRIM gene cluster. Alternative splicing results in multiple transcript variants. A read-through transcript from this gene into the downstream TRIM34 gene has also been observed, which results in a fusion product from these neighboring family members.

References

1. Reymond, A., Meroni, G., Fantozzi, A., Merla, G., Cairo, S., Luzi, L., Riganelli, D., Zanaria, E., Messali, S., Cainarca, S., Guffanti, A., Minucci, S., Pelicci, P. G., Ballabio, A. The tripartite motif family identifies cell compartments. EMBO J. 20: 2140-2151, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 310772223
• NCBI GeneID: 117854
• NCBI Accession #: NP_001185574.1
• NCBI GenBank Nucleotide #: NP_001185574.1
• UniProt Accession #: Q9C030
• NCBI Official Full Name: tripartite motif-containing protein 6 isoform 3
• NCBI Official Synonym Full Names: tripartite motif containing 6
• NCBI Official Symbol: TRIM6
• NCBI Official Synonym Symbols: RNF89
• NCBI Protein Information: tripartite motif-containing protein 6
• UniProt Protein Name: Tripartite motif-containing protein 6
• UniProt Gene Name: TRIM6
• UniProt Synonym Gene Names: RNF89