Rabbit COX IV/COX4I1 Polyclonal Antibody | anti-COX4I1 antibody

Anti-COX IV/COX4I1 Antibody Picoband

Cat. Num. AAA19549

• Gene Names: COX4I1; COX4; COXIV; COX4-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: COX IV/COX4I1, Antibody; Anti-COX IV/COX4I1 Antibody Picoband; anti-COX4I1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-COX4I1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 0.25-0.5 ug/ml, Human, Mouse, Rat; IHC-P: 2-5 ug/ml, Human, Mouse, Rat; Tested Species: In-house tested species with positive results.; Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: A synthetic peptide corresponding to a sequence of COX IV/COX4I1 (EVAHVKHLSASQKALKEK).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Figure 8. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 7. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot)

(Figure 2. Western blot analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat skeletal muscle tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat stomach tissue lysates,Lane 4: rat small intestine tissue lysates,Lane 5: mouse skeletal muscle tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse stomach tissue lysates,Lane 8: mouse small intestine tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV/COX4I1 antigen affinity purified polyclonal antibody (#AAA19549) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV/COX4I1 at approximately 17 kDa. The expected band size for COX IV/COX4I1 is at 17 kDa.)

WB (Western Blot)

(Figure 1. Western blot analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human MOLT4 whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human Hela whole cell lysates,Lane 6: human Caco-2 whole cell lysates,Lane 7: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV/COX4I1 antigen affinity purified polyclonal antibody (#AAA19549) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV/COX4I1 at approximately 17 kDa. The expected band size for COX IV/COX4I1 is at 17 kDa.)

Related Product Information for anti-COX4I1 antibody

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial is an enzyme that in humans is encoded by the COX4I1 gene. Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14.

Product Categories/Family for anti-COX4I1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502981
• NCBI GeneID: 1327
• NCBI Accession #: NP_001852.1
• NCBI GenBank Nucleotide #: NM_001861.3
• UniProt Accession #: P13073; Q6P666; B2R4J2; D3DUM7
• Molecular Weight: 19,577 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit IV isoform 1
• NCBI Official Symbol: COX4I1
• NCBI Official Synonym Symbols: COX4; COXIV; COX4-1
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX IV-1; cytochrome c oxidase polypeptide IV
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Gene Name: COX4I1
• UniProt Synonym Gene Names: COX4; COX IV-1
• UniProt Entry Name: COX41_HUMAN