Rabbit RBP1 Polyclonal Antibody | anti-RBP1 antibody

Anti-RBP1 Antibody Picoband

Cat. Num. AAA19800

• Gene Names: RBP1; CRBP; RBPC; CRBP1; CRBPI; CRABP-I
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: RBP1, Antibody; Anti-RBP1 Antibody Picoband; Heterogeneous nuclear ribonucleoprotein L; ubiquitin like modifier activating enzyme 2; SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115; anti-RBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Duodenum, heart, lung and liver, but not thymus.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-RBP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml, Human, Rat; IHC(Paraffin-embedded Section): 2-5ug/ml, Human, Mouse, Rat; ICC/IF: 5ug/ml, Human; FC/FACS (Fixed): 1-3ug/1x10⁶ cells, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human RBP1 recombinant protein (Position: K10-Q135).
• Subcellular Localization: Cytoplasm, Nucleus. Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins.
• Protein Function: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 8. Flow Cytometry analysis of U2OS cells using anti-RBP1 antibody (AAA19800).Overlay histogram showing U2OS cells stained with AAA19800 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBP1 Antibody (AAA19800, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence)

(Figure 7. IF analysis of RBP1 using anti-RBP1 antibody (AAA19800) and anti-Tubulin Alpha antibody (M03989-3).RBP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP1 antigen affinity purified polyclonal antibody (#AAA19800) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBP1 at approximately 16 kDa. The expected band size for RBP1 is at 16 kDa.)

Related Product Information for anti-RBP1 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-RBP1 antibody

Primary Antibodies; Cell Biology; Proteasome/Ubiquitin; Proteolysis/Ubiquitin

References

1. Colantuoni, V., Cortese, R., Nilsson, M., Lundvall, J., Bavik, C. O., Eriksson, U., Peterson, P. A., Sundelin, J. Cloning and sequencing of a full length cDNA corresponding to human cellular retinol-binding protein. Biochem. Biophys. Res. Commun. 130: 431-439, 1985.2. Colantuoni, V., Rocchi, M., Roncuzzi, L., Romeo, G. Mapping of human cellular retinol-binding protein to chromosome 3. Cytogenet. Cell Genet. 43: 221-222, 1986. 3. De Baere, E., Speleman, F., Van Roy, N., De Paepe, A., Messiaen, L. Assignment of the cellular retinol-binding protein 1 gene (RBP1) and of the coatomer beta-prime subunit gene (COPB2) to human chromosome band 3q23 by in situ hybridization. Cytogenet. Cell Genet. 82: 226-227, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 195976809
• NCBI GeneID: 5947
• NCBI Accession #: NP_001124464.1
• NCBI GenBank Nucleotide #: NM_001130992.1
• UniProt Accession #: P09455; Q6FGX8; A8K2Q0; B7Z7A0; E7EWV0; F2Z2F2
• Molecular Weight: 17,523 Da
• NCBI Official Full Name: retinol-binding protein 1 isoform b
• NCBI Official Synonym Full Names: retinol binding protein 1, cellular
• NCBI Official Symbol: RBP1
• NCBI Official Synonym Symbols: CRBP; RBPC; CRBP1; CRBPI; CRABP-I
• NCBI Protein Information: retinol-binding protein 1; CRBP-I; cellular retinol-binding protein I; retinol-binding protein 1, cellular
• UniProt Protein Name: Retinol-binding protein 1
• UniProt Gene Name: RBP1
• UniProt Synonym Gene Names: CRBP1; CRBP; CRBP-I
• UniProt Entry Name: RET1_HUMAN