Human Agrin ELISA Kit | Agrin elisa kit
Human Agrin ELISA Kit
Gene Names
AGRN; CMSPPD
Reactivity
Human
Synonyms
Agrin; N/A; Human Agrin ELISA Kit; Agrin elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for Agrin elisa kit
Intended Uses: This AGR-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGR-Ab. This ELISA kit for research use only!
Principle of the Assay: AGR-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a AGR antigen and an AGR-Ab-HRP conjugate. The assay sample and buffer are incubated together with AGR-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGR-Ab concentration since AGR-Ab from samples and AGR-Ab-HRP conjugate compete for the AGR antigen binding site. Since the number of sites is limited, as more sites are occupied by AGR-Ab from the sample, fewer sites are left to bind AGR-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGR-Ab concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AGR-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a AGR antigen and an AGR-Ab-HRP conjugate. The assay sample and buffer are incubated together with AGR-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGR-Ab concentration since AGR-Ab from samples and AGR-Ab-HRP conjugate compete for the AGR antigen binding site. Since the number of sites is limited, as more sites are occupied by AGR-Ab from the sample, fewer sites are left to bind AGR-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGR-Ab concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Agrin elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
216,733 Da
NCBI Official Full Name
agrin, partial
NCBI Official Synonym Full Names
agrin
NCBI Official Symbol
AGRN
NCBI Official Synonym Symbols
CMSPPD
NCBI Protein Information
agrin; agrin proteoglycan
UniProt Protein Name
Agrin
UniProt Gene Name
AGRN
UniProt Synonym Gene Names
AGRIN; C90; C22
UniProt Entry Name
AGRIN_HUMAN
