Rabbit Caspase 1 (CASP1) Polyclonal Antibody | anti-CASP1 antibody

Polyclonal Antibody to Caspase 1 (CASP1)

Cat. Num. AAA20089

• Reactivity: Sus scrofa; Porcine (Pig)
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Caspase 1 (CASP1); Polyclonal Antibody; Polyclonal Antibody to Caspase 1 (CASP1); anti-CASP1 antibody

• Host: Rabbit
• Reactivity: Sus scrofa; Porcine (Pig)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CASP1 antibody: Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL;; Immunohistochemistry: 5-20ug/mL;; Immunocytochemistry: 5-20ug/mL;; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Human
• Immunogen: Recombinant CASP1 (Asn120~Asp297) expressed in E.coli
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

IF (Immunofluorescence)

(FITC staining on IF;Sample: Human U2OS cell;Primary Ab: 30ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: Porcine Small intestine Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: Porcine Cerebrum Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistchemistry)

(DAB staining on IHC-P;Sample: Human Placenta Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Samples: Porcine Small intestine Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Samples: Porcine Pancreas Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistchemistry)

(DAB staining on IHC-P;Samples: Porcine Spleen Tissue;Primary Ab: 10ug/ml Rabbit Anti- Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Samples: Porcine Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Samples: Porcine Cardiac Muscle Tissue;Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Knockout Verification

(Knockout Varification:Lane 1: Wild-type Raji cell lysate;Lane 2: CASP1 knockout Raji cell lysate;Predicted MW: 10,30,35,43,45kDaObserved MW: 42kDaPrimary Ab: 5ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot)

(Sample:Lane1: Porcine Spleen lysate;Lane2: Porcine Lung lysate;Lane3: Raji cell lysatePrimary Ab: 1ug/ml Rabbit Anti-Porcine CASP1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot)

(Sample: Recombinant CASP1, Porcine.)

Product Categories/Family for anti-CASP1 antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 47523412
• NCBI GeneID: 397319
• NCBI Accession #: NP_999327.1
• NCBI GenBank Nucleotide #: NM_214162.1
• UniProt Accession #: Q9N2I1
• NCBI Official Full Name: caspase-1
• NCBI Official Symbol: CASP1
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme