Enolase, Muscle Specific (MSE) Recombinant Protein | MSE recombinant protein
Recombinant Enolase, Muscle Specific (MSE)
Gene Names
ENO3; ENO1
Reactivity
Oryctolagus cuniculus (Rabbit)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Enolase, Muscle Specific (MSE); N/A; Recombinant Enolase, Muscle Specific (MSE); MSE recombinant protein
Host
E.coli
Reactivity
Oryctolagus cuniculus (Rabbit)
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
MQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKSRYLGK GVLKAVEHIN KTLGPALLEK KLSVVDQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN HDLVLPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFRE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FHRNGKYDLD FKSPDDPARH ITGQKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VDIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AVFAGRKFRN PKAK
Applicable Applications for MSE recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Met3~Lys434
Tags
N-terminal His Tag
Subcellular Location
Chromosome
Traits
Freeze-dried powder
Predicted isoelectric point
7.3
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
Gene Sequencing (Extract)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 48.6kDa
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
beta-enolase
NCBI Official Symbol
ENO3
NCBI Official Synonym Symbols
ENO1
NCBI Protein Information
beta-enolase; MSE; beta beta enolase; enolase 1, (alpha); muscle-specific enolase; skeletal muscle enolase; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Beta-enolase
UniProt Gene Name
ENO3
UniProt Synonym Gene Names
MSE
UniProt Entry Name
ENOB_RABIT
