Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Recombinant Protein | WNT5A recombinant protein
Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Gene Names
Wnt5a; Wnt-5a; 8030457G12Rik
Reactivity
Mouse
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
Purity
> 95%
Synonyms
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A); N/A; Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A); WNT5A recombinant protein
Host
E Coli
Reactivity
Mouse
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Concentration
200ug/mL (varies by lot)
Applicable Applications for WNT5A recombinant protein
SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Ile62~Lys380
Tags
N-terminal GST-tag
Tissue Specificity
Cerebellum
Subcellular Location
Secreted, extracellular space, extracellular matrix
Traits
Freeze-dried powder
Predicted isoelectric point
8.8
Predicted Molecular Mass
61.8kDa
Accurate Molecular Mass
62kDa as determined by SDS-PAGE reducing conditions
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
SDS-PAGE
Sequence Information
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein Wnt-5a isoform 2
NCBI Official Synonym Full Names
wingless-related MMTV integration site 5A
NCBI Official Symbol
Wnt5a
NCBI Official Synonym Symbols
Wnt-5a; 8030457G12Rik
NCBI Protein Information
protein Wnt-5a
UniProt Protein Name
Protein Wnt-5a
UniProt Gene Name
Wnt5a
UniProt Synonym Gene Names
Wnt-5a
UniProt Entry Name
WNT5A_MOUSE
