Human Wingless Type MMTV Integration Site Family, Member 1 (WNT1) ELISA Kit | WNT1 elisa kit
Human Wingless Type MMTV Integration Site Family, Member 1 (WNT1) ELISA Kit
Reactivity
Human
Synonyms
Wingless Type MMTV Integration Site Family, Member 1 (WNT1); N/A; Human Wingless Type MMTV Integration Site Family, Member 1 (WNT1) ELISA Kit; WNT1 elisa kit
Reactivity
Human
Sequence Length
372
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 30ng/ml
Sensitivity
0.02ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for WNT1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Wingless Type MMTV Integration Site Family, Member 1 (also known as WNT1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human WNT1 antibody. WNT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human WNT1 Antibody is added and binds to WNT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human WNT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human WNT1 antibody. WNT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human WNT1 Antibody is added and binds to WNT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human WNT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
wingless-type MMTV integration site family
NCBI Official Symbol
WntB
NCBI Protein Information
wingless-type MMTV integration site family
