Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit | TMPRSS2 elisa kit
Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human
Synonyms
Transmembrane Protease, Serine 2 (TMPRSS2); N/A; Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit; PRSS10; Serine protease 10; TMPRSS2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TMPRSS2. No significant cross-reactivity or interference between TMPRSS2 and analogues was observed.
Sequence Length
492
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.055ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TMPRSS2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TMPRSS2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for TMPRSS2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TMPRSS2 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TMPRSS2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TMPRSS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TMPRSS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TMPRSS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TMPRSS2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TMPRSS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TMPRSS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TMPRSS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TMPRSS2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,690 Da
NCBI Official Full Name
Transmembrane protease, serine 2
NCBI Official Synonym Full Names
transmembrane protease, serine 2
NCBI Official Symbol
TMPRSS2
NCBI Official Synonym Symbols
PP9284; PRSS10
NCBI Protein Information
transmembrane protease serine 2
UniProt Protein Name
Transmembrane protease serine 2
UniProt Gene Name
TMPRSS2
UniProt Synonym Gene Names
PRSS10
UniProt Entry Name
TMPS2_HUMAN
