Mannose Associated Serine Protease 2 (MASP2) Active Protein | MASP2 active protein

Active Mannose Associated Serine Protease 2 (MASP2)

Cat. Num. AAA21058

• Gene Names: MASP2; sMAP; MAP19; MASP-2; MASP1P1
• Reactivity: Homo sapiens (Human)
• Applications: Cell culture; Activity Assays; In vivo assays.
• Purity: >98%
• Synonyms: Mannose Associated Serine Protease 2 (MASP2); Active Mannose Associated Serine Protease 2 (MASP2); Mannose-binding protein-associated serine protease 2; mannan-Binding Lectin Serine Peptidase 2; MBL-associated serine protease 2; MASP2 active protein

• Host: E. coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: >98%
• Form/Format: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 1000ug/mL (varies by lot)

• Applicable Applications for MASP2 active protein: Cell culture; Activity Assays; In vivo assays.
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Ile445~Ile683
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted isoelectric point: 5.7
• Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Sample: Recombinant MASP2, Human;Antibody: Rabbit Anti-Human MASP2 Ab)

SDS-PAGE

(Sample: Active recombinant MASP2, Human)

Gene Sequencing (extract)

Activity

(MASP2 (Mannan-binding lectin serine protease 2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. The preproprotein of MASP2 is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease, which is able to associate with MBL2. Thus, a functional binding ELISA assay was constructed to detect the association of rhMASP2 with MBL2. Briefly, rhMASP-2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MBL2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MASP-2 pAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MASP2 with MBL2 was shown in Figure 1 and this effect was in a dose dependent manner.)

Sequence Information

Product Categories/Family for MASP2 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 21264363
• NCBI GeneID: 10747
• NCBI Accession #: NP_006601.2
• NCBI GenBank Nucleotide #: NM_006610.3
• UniProt Accession #: O00187; O75754; Q5TEQ5; Q5TER0; Q96QG4; Q9BZH0; Q9H498; Q9H499; Q9UBP3; Q9UC48; A8K458; A8MWJ2
• Molecular Weight: Predicted Molecular Mass: 27.4kDa; Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: mannan-binding lectin serine protease 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: mannan binding lectin serine peptidase 2
• NCBI Official Symbol: MASP2
• NCBI Official Synonym Symbols: sMAP; MAP19; MASP-2; MASP1P1
• NCBI Protein Information: mannan-binding lectin serine protease 2
• UniProt Protein Name: Mannan-binding lectin serine protease 2
• UniProt Gene Name: MASP2
• UniProt Synonym Gene Names: MASP-2