Mouse Interleukin 10 (IL10) ELISA Kit | IL10 elisa kit

High Sensitivity Mouse Interleukin 10 (IL10) ELISA Kit

Cat. Num. AAA20553

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Reactivity: Mouse
• Synonyms: Interleukin 10 (IL10); High Sensitivity Mouse Interleukin 10 (IL10) ELISA Kit; CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor; IL10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL10. No significant cross-reactivity or interference between IL10 and analogues was observed.
• Sequence Length: 178
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Detection Range: 1.56-100pg/mL
• Sensitivity: 0.44pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for IL10 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL10 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL10. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL10 elisa kit

Cytokine; Infection immunity

References

miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice; Comput Math Methods Med; 2022 Jul 20;2022:9088727.; PMID: 35912153; Guotao Zou; Department of Pediatrics, Yongchuan Hospital of Chongqing Medical University, 439 Xuanhua Road, Yongchuan District, Chongqing, China 402160.

NCBI and Uniprot Product Information

• NCBI GI #: 49457009
• NCBI GeneID: 3586
• UniProt Accession #: Q6FGS9; P22301
• Molecular Weight: 20,493 Da
• NCBI Official Full Name: IL10, partial
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: IL10 protein
• UniProt Gene Name: IL10
• UniProt Entry Name: Q6FGS9_HUMAN