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ChIP (Chromatin Immunoprecipitation)
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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (AAA10650) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

H3K9me3 Antibody | anti-H3K9me3 antibody

Histone H3K9me3 Polyclonal Antibody

Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Dot Blot, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
H3K9me3; N/A; Histone H3K9me3 Polyclonal Antibody; TriMethyl-Histone H3-K9: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3; anti-H3K9me3 antibody
Ordering
For Research Use Only!
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Sequence Length
136
Applicable Applications for anti-H3K9me3 antibody
Dot Blot (DB), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), CHIP
Application Notes
DB 1:500 - 1:2000
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:50 - 1:200
Source
Human
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Species Reactivity/Cross Reactivity
Human, Mouse, Rat, Other (Wide Range)
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

ChIP (Chromatin Immunoprecipitation)

(Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (AAA10650) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (AAA10650) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IHC (Immunohistochemistry)

(mmunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (AAA10650) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (mmunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (AAA10650) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (AAA10650) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (AAA10650) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human liver cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human kidney using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).)

DB (Dot Blot)

(Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.)

DB (Dot Blot) (Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Related Product Information for anti-H3K9me3 antibody
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Product Categories/Family for anti-H3K9me3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 17KDa
Calculated MW: 15kDa
NCBI Official Full Name
Histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN

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Product Notes

The H3K9me3 hist3h3 (Catalog #AAA10650) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's H3K9me3 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), CHIP. DB 1:500 - 1:2000 WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:50 - 1:200. Researchers should empirically determine the suitability of the H3K9me3 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3K9me3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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