Rabbit anti-Human Apolipoprotein A4 Antibody | anti-APOA4 antibody
Polyclonal Antibody to Apolipoprotein A4 (APOA4)
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Apolipoprotein A4; N/A; Polyclonal Antibody to Apolipoprotein A4 (APOA4); anti-APOA4 antibody
Host
Rabbit
Reactivity
Human
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.42mg/ml (varies by lot)
Applicable Applications for anti-APOA4 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Porcine
Immunogen
Recombinant APOA4 (Asp173~Ser396) expressed in E.coli
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Small intestine Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human APOA4 AntibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Human Serum;Lane 2: Human Lung lysatePrimary Ab: 3ug/ml Rabbit Anti-Human APOA4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Recombinant APOA4, Human.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Apolipoprotein A-IV
NCBI Official Synonym Full Names
apolipoprotein A4
NCBI Official Symbol
APOA4
NCBI Protein Information
apolipoprotein A-IV
UniProt Protein Name
Apolipoprotein A-IV
UniProt Gene Name
APOA4
UniProt Synonym Gene Names
Apo-AIV; ApoA-IV
