Mouse Apolipoprotein B48 ELISA Kit | Apo-B48 elisa kit
Mouse Apolipoprotein B48 ELISA Kit
Gene Names
APOB; FLDB; LDLCQ4
Reactivity
Mouse
Synonyms
Apolipoprotein B48; N/A; Mouse Apolipoprotein B48 ELISA Kit; Apo-B48 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ApoB48. No significant cross-reactivity or interference between ApoB48 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoB48 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ?g/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for Apo-B48 elisa kit
Intended Uses: This ApoB48 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ApoB48. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ApoB48 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ApoB48 antibody and an ApoB48-HRP conjugate. The assay sample and buffer are incubated together with ApoB48-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoB48 concentration since ApoB48 from samples and ApoB48-HRP conjugate compete for the anti-ApoB48 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoB48 from the sample, fewer sites are left to bind ApoB48-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoB48 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ApoB48 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ApoB48 antibody and an ApoB48-HRP conjugate. The assay sample and buffer are incubated together with ApoB48-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoB48 concentration since ApoB48 from samples and ApoB48-HRP conjugate compete for the anti-ApoB48 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoB48 from the sample, fewer sites are left to bind ApoB48-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoB48 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Apo-B48 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
515,605 Da
NCBI Official Full Name
apolipoprotein B-48, partial
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
APOB
NCBI Official Synonym Symbols
FLDB; LDLCQ4
NCBI Protein Information
apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
UniProt Protein Name
Apolipoprotein B-100
UniProt Gene Name
APOB
UniProt Synonym Gene Names
Apo B-100; Apo B-48
UniProt Entry Name
APOB_HUMAN
