Standard Curve (Sample)
Mouse Interleukin 4 (IL4) ELISA Kit | IL4 elisa kit
Mouse Interleukin 4 (IL4) Wide-range ELISA Kit
Gene Names
Il4; Il-4; BSF-1
Reactivity
Mouse
Applications
ELISA
Synonyms
Interleukin 4 (IL4); Mouse Interleukin 4 (IL4) Wide-range ELISA Kit; BSF1; BCGF1 ; B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra; IL4 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of IL4. No significant cross-reactivity or interference between IL4 and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
31.2-2,000pg/mL
Sensitivity
12.7pg/mL
Applicable Applications for IL4 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked Immunosorbent Assay for Antigen Detection.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL4 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL4 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for IL4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,834 Da
NCBI Official Full Name
interleukin-4
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
Il4
NCBI Official Synonym Symbols
Il-4; BSF-1
NCBI Protein Information
interleukin-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; IGG1 induction factor; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
UniProt Gene Name
Il4
UniProt Synonym Gene Names
Il-4; IL-4; BSF-1
UniProt Entry Name
IL4_MOUSE
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Product Notes
The Mouse IL4 il4 (Catalog #AAA21170) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA21170 ELISA Kit recognizes Mouse IL4. AAA Biotech's Interleukin 4 (IL4) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked Immunosorbent Assay for Antigen Detection. Researchers should empirically determine the suitability of the IL4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 4 (IL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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