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WB (Western Blot)
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Rabbit anti-Human Pyruvate kinase isozymes M2 (PKM2) Polyclonal Antibody | anti-PKM2 antibody

Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Pyruvate kinase isozymes M2 (PKM2), Antibody; Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2); Pyruvate kinase isozymes M2; M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle; anti-PKM2 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Met1~Pro531
Applicable Applications for anti-PKM2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:250-2500
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
Conjugation
Unconjugated
Research Area
Enzyme & Kinase, Tumor immunity
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: K562 cell lysate; Lane4: MCF7 cell lysatePrimary Ab: 0.2ug/ml Rabbit Anti-Human PKM2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.2ug/ml Rabbit Anti-Human PKM2 Antibody Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IF (Immunofluorescence)

(AF488 staining on IF;Sample: Hela cellPrimary Ab: 20ug/ml Rabbit Anti-Human PKM2 AntibodySecond Ab: 2ug/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin)

(DAB staining on IHC-P;Sample: Mouse Kidney TissuePrimary Ab: 10ug/ml Rabbit Anti-Human PKM2 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin)

(DAB staining on IHC-P;Sample: Mouse Lung TissuePrimary Ab: 10ug/ml Rabbit Anti-Human PKM2 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin)

(DAB staining on IHC-P;Sample: Mouse Testis TissuePrimary Ab: 10ug/ml Rabbit Anti-Human PKM2 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Product Categories/Family for anti-PKM2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
531
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1
NCBI Protein Information
pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; th
UniProt Protein Name
Pyruvate kinase PKM
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN

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Product Notes

The PKM2 pkm (Catalog #AAA21265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate kinase isozymes M2 (PKM2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:250-2500 IHC: 5-20ug/mL; 1:25-100 ICC: 5-20ug/mL; 1:25-100. Researchers should empirically determine the suitability of the PKM2 pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Pro53 1. It is sometimes possible for the material contained within the vial of "Pyruvate kinase isozymes M2 (PKM2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.