IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
Rabbit anti-Human, Pig Pyruvate Dehydrogenase Complex Component X Polyclonal Antibody | anti-PDHX antibody
Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRQDLV KDDIKVSVND FIIKAAAVTL KQMPDVNVSW DGEGPKQLPF IDISVAVATD KGLLTPIIKD AAAKGIQEIA DSVKALSKKA RDGKLLPEEY QGGSFSISNL
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)
IHC (Immunohistochemistry)
(FITC staining on IHC-P; Samples: Human Hela Cells)
IHC (Immunohistochemistry)
(FITC staining on IHC-P; Samples: Human Hela Cells)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
WB (Western Blot)
(Western Blot: Sample: Recombinant PDHX, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant PDHX, Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Heart Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Heart Tissue.)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PDHX pdhx (Catalog #AAA20946) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX) reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Dehydrogenase Complex Component X can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PDHX pdhx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRQDLV KDDIKVSVND FIIKAAAVTL KQMPDVNVSW DGEGPKQLPF IDISVAVATD KGLLTPIIKD AAAKGIQEIA DSVKALSKKA RDGKLLPEEY QGGSFSISNL. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase Complex Component X, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is rabbit anti-human antibody?
Rabbit anti-human antibody is a secondary antibody produced in rabbits that specifically recognizes and binds to human immunoglobulins and proteins. These polyclonal antibodies are widely used in immunoassays, western blotting, immunofluorescence, and flow cytometry for detecting human antigens.
What is a rabbit anti-human antibody used for in laboratory applications?
Rabbit anti-human antibodies detect human proteins and immunoglobulins in ELISA, western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. They're essential for diagnostic assays, research applications measuring human biomarkers, and therapeutic antibody characterization in pharmaceutical development.
What is the difference between rabbit IgG and human IgG?
Rabbit IgG is a secondary antibody produced in rabbits used to detect human antigens. Human IgG is a primary target protein representing human immune globulin. Rabbit anti-human antibodies specifically recognize the constant and variable regions of human IgG molecules for immunoassay applications.
Is this antibody affinity-purified or cross-adsorbed for higher specificity?
Premium rabbit anti-human antibodies are typically affinity-purified against immobilized human IgG, removing non-specific binding. Cross-adsorbed variants are pre-treated to remove reactivity against other species, reducing background in multiplex assays. Verify purification method with product specifications for your application requirements.
What storage conditions are required to maintain antibody stability?
Rabbit anti-human antibodies are typically stored at 2-8°C in original vials with preservatives (sodium azide or similar). Long-term storage requires -20°C conditions. Avoid freeze-thaw cycles beyond 3-5 iterations, maintain sterile conditions, and keep containers tightly sealed to prevent contamination and degradation.
What conjugate formats are available for this antibody (HRP, FITC, Alexa Fluor, etc.)?
Rabbit anti-human antibodies are available in unconjugated, HRP (horseradish peroxidase), FITC (fluorescein), Alexa Fluor (488, 555, 647), biotin, and alkaline phosphatase formats. Selection depends on detection platform (ELISA, western blot, fluorescence microscopy, flow cytometry) and compatibility with available instrumentation.
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