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IHC (Immunohistchemistry) (KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human lung using KPNA2 antibody at dilution of 1:200 (200x lens).)

Rabbit anti-Mouse, Human KPNA2/Importin Alpha 1 Polyclonal Antibody | anti-KPNA2 antibody

KPNA2/Importin Alpha 1 Rabbit anti-Human Polyclonal Antibody

Gene Names
KPNA2; QIP2; RCH1; IPOA1; SRP1alpha
Reactivity
Mouse, Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity purified
Synonyms
KPNA2/Importin Alpha 1; Polyclonal Antibody; KPNA2/Importin Alpha 1 Rabbit anti-Human Polyclonal Antibody; IHC-plus KPNA2/Importin Alpha 1 Antibody; KPNA2; Importin alpha 2; Importin subunit alpha-2; IPOA1; Importin alpha-2 subunit; Karyopherin subunit alpha-2; Importin-alpha-P1; Qip2; RAG cohort 1; SRP1; RCH1; SRP1-alpha; SRP1alpha; Importin alpha 1; Pendulin; RAG cohort protein 1; anti-KPNA2 antibody
Ordering
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human KPNA2/Importin Alpha 1
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol
Applicable Applications for anti-KPNA2 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IF: 1:50-1:200
IHC: 1:50-1:200
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 57kDa, while the observed MW by Western blot was 60kDa.
Target
Human KPNA2/Importin Alpha 1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human KPNA2 (NP_002257.1). MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVV
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.

IHC (Immunohistchemistry)

(KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human lung using KPNA2 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistchemistry) (KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human lung using KPNA2 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry)

(KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human kidney using KPNA2 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human kidney using KPNA2 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry)

(KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded rat brain using KPNA2 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded rat brain using KPNA2 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistchemistry)

(KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using KPNA2 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry) (KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using KPNA2 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry)

(KPNA2/Importin Alpha 1 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (KPNA2/Importin Alpha 1 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(KPNA2/Importin Alpha 1 Antibody-Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody. Western blot was performed from the immunoprecipitate using KPNA2 antibodyat a dilition of 1:1000.)

WB (Western Blot) (KPNA2/Importin Alpha 1 Antibody-Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody. Western blot was performed from the immunoprecipitate using KPNA2 antibodyat a dilition of 1:1000.)

WB (Western Blot)

(KPNA2/Importin Alpha 1 Antibody-Western blot analysis of extracts of various cell lines, using KPNA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)

WB (Western Blot) (KPNA2/Importin Alpha 1 Antibody-Western blot analysis of extracts of various cell lines, using KPNA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)

ICC (Immunocytochemistry)

(KPNA2/Importin Alpha 1 Antibody-Immunofluorescence analysis of A549 cells.)

ICC (Immunocytochemistry) (KPNA2/Importin Alpha 1 Antibody-Immunofluorescence analysis of A549 cells.)

ICC (Immunocytochemistry)

(KPNA2/Importin Alpha 1 Antibody-Immunofluorescence analysis of MCF-7 cells using KPNA2 antibody. Blue: DAPI for nuclear staining.)

ICC (Immunocytochemistry) (KPNA2/Importin Alpha 1 Antibody-Immunofluorescence analysis of MCF-7 cells using KPNA2 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-KPNA2 antibody
Importin Alpha 1 antibody is an unconjugated rabbit polyclonal antibody to Importin Alpha 1 (KPNA2) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,862 Da
NCBI Official Full Name
importin subunit alpha-1
NCBI Official Synonym Full Names
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
NCBI Official Symbol
KPNA2
NCBI Official Synonym Symbols
QIP2; RCH1; IPOA1; SRP1alpha
NCBI Protein Information
importin subunit alpha-1; RAG cohort 1; RAG cohort protein 1; SRP1-alpha; importin alpha 1; importin alpha 2; importin subunit alpha-2; importin-alpha-P1; karyopherin subunit alpha-2; pendulin
UniProt Protein Name
Importin subunit alpha-1
UniProt Gene Name
KPNA2
UniProt Synonym Gene Names
RCH1; SRP1
UniProt Entry Name
IMA1_HUMAN

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Product Notes

The KPNA2 kpna2 (Catalog #AAA21307) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KPNA2/Importin Alpha 1 Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's KPNA2/Importin Alpha 1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB). IF: 1:50-1:200 IHC: 1:50-1:200 IHC-P: 1:200 WB: 1:500-1:2000 The predicted MW is 57kDa, while the observed MW by Western blot was 60kDa. Researchers should empirically determine the suitability of the KPNA2 kpna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KPNA2/Importin Alpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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