Rabbit ANXA1/Annexin A1 Polyclonal Antibody | anti-ANXA1 antibody
ANXA1/Annexin A1 Rabbit anti-Human Polyclonal (Internal) Antibody
IHC (Immunohistochemistry)
(ANXA1/Annexin A1 Antibody-Immunohistochemical analysis of Annexin A1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)
IHC (Immunohistchemistry)
(ANXA1/Annexin A1 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(ANXA1/Annexin A1 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(ANXA1/Annexin A1 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(ANXA1/Annexin A1 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(ANXA1/Annexin A1 Antibody-Western blot analysis of Annexin A1 expression in HEK293T (A); NIH3T3 (B); THP1 (C); Molt4 (D); HepG2 (E) whole cell lysates.)
ICC (Immunocytochemistry)
(ANXA1/Annexin A1 Antibody-Immunofluorescent analysis of Annexin A1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
