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IHC (Immunohistchemistry)
(PPIF/Cyclophilin F Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human PPIF/Cyclophilin F Monoclonal Antibody | anti-PPIF antibody
PPIF/Cyclophilin F Mouse anti-Human Monoclonal (OTI7D12) Antibody
Gene Names
PPIF; CYP3; CypD; CyP-M; Cyp-D
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
PPIF/Cyclophilin F; Monoclonal Antibody; PPIF/Cyclophilin F Mouse anti-Human Monoclonal (OTI7D12) Antibody; IHC-plus PPIF/Cyclophilin F Antibody (clone OTI7D12); PPIF; Cyclophilin 3; CYP3; Cyp-D; HCyP3; Peptidylprolyl isomerase F; Rotamase F; PPIase F; Cyclophilin D; Cyclophilin F; anti-PPIF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
OTI7D12
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PPIF antibody
Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Target
Human PPIF/Cyclophilin F
Immunogen
Full length human recombinant protein of human PPIF(NP_005720) produced in E.coli.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistchemistry)
(PPIF/Cyclophilin F Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(PPIF/Cyclophilin F Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(PPIF/Cyclophilin F Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(PPIF/Cyclophilin F Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-Western blot analysis of extracts. (35ug) from mouse heart tissue lysate by using anti-PPIF monoclonal antibody. (1:500))
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-Western blot analysis of extracts. (35ug) from mouse heart tissue lysate by using anti-PPIF monoclonal antibody. (1:500))
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY PPIF. (Right lane) cDNA for 48 hrs and lysed)
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY PPIF. (Right lane) cDNA for 48 hrs and lysed)
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-Western blot analysis of extracts. (35ug) from 4 different cell lines by using anti-PPIF monoclonal antibody. (1:500))
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-Western blot analysis of extracts. (35ug) from 4 different cell lines by using anti-PPIF monoclonal antibody. (1:500))
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-Equivalent amounts of cell lysates and PPIF-Knockout 293T cells were separated by SDS-PAGE and immunoblotted with anti-PPIF monoclonal antibody(1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.)
WB (Western Blot)
(PPIF/Cyclophilin F Antibody-Equivalent amounts of cell lysates and PPIF-Knockout 293T cells were separated by SDS-PAGE and immunoblotted with anti-PPIF monoclonal antibody(1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.)
Related Product Information for anti-PPIF antibody
Cyclophilin F antibody is an unconjugated mouse monoclonal antibody to human Cyclophilin F (PPIF). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,040 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F
NCBI Official Symbol
PPIF
NCBI Official Synonym Symbols
CYP3; CypD; CyP-M; Cyp-D
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; rotamase F; cyclophilin 3; cyclophilin D; cyclophilin F; mitochondrial cyclophilin; peptidyl-prolyl cis-trans isomerase, mitochondrial
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Gene Name
PPIF
UniProt Synonym Gene Names
CYP3; PPIase F; CyP-D; CypD; CyP-M
UniProt Entry Name
PPIF_HUMAN
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Product Notes
The PPIF ppif (Catalog #AAA21376) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPIF/Cyclophilin F Mouse anti-Human Monoclonal (OTI7D12) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPIF/Cyclophilin F can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIF/Cyclophilin F, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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